Proteomic Identification Principles for Fine Animal Fiber Verification
LC-MS/MS tryptic peptide analysis provides species-specific verification of fine animal fibers, overriding optical scale degradation in processed blends.
MRM Transition
Targeted Detection
Specific combinations of precursor and fragment ion masses are monitored in tandem mass spectrometry to identify known target compounds in complex mixtures. The selection of a specific mrm transition provides high sensitivity and selectivity for detecting diagnostic peptides in hair protein digests. This approach filters out background noise from non-target proteins.
Transition Parameter
The primary precursor ion is isolated in the first mass analyzer and fragmented by collision with an inert gas. In an mrm transition, only a specific fragment ion is allowed to pass to the final detector. This double selection ensures that the observed signal comes solely from the target peptide of interest.
Fibre Screening
Testing facilities set up these transition parameters to scan quickly for the presence of cashmere, yak or sheep wool in blended yarns. This targeted method allows for the rapid screening of dozens of samples per day with high precision. It is used by quality control laboratories to verify raw material composition before starting bulk garment production.
Suppliers who provide falsified materials can be quickly identified and audited using these specific mass signals.
Assay Performance
The mass spectrometer records a clear chromatogram peak with an intensity directly proportional to the target peptide concentration. Monitoring each mrm transition delivers a rapid, reliable and low-cost method for verifying luxury fibre blends.